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. 2023 Jun 29;55(7):1149–1163. doi: 10.1038/s41588-023-01424-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 10 — ((a-c) In situ hybridization staining for Isl1 (a), Gata2 (b), and Dnajb8 (c) expression in wild type (left column) and cRE1^dup/+ (right column) embryos at the indicated ages and axial levels. In the cRE1^dup/+ embryos, Isl1 and Gata2 expression was expanded in r4, and fewer Isl1⁺ FBMNs were detected in r6 at E14.5. Dnajb8 expression was not detected in the hindbrain region of developing r4, but the same probe did detect robust and specific expression in developing spermatids in adult WT and cRE1^dup/+ testis⁸⁸. n = 2 (E10.5), 4 (E12.5), 2 (E14.5), 3 (adult testes) samples each for WT and cRE1^dup/+. Arrows mark OCNs, arrowheads mark FBMNs. All scale bars = 200um and apply to all panels with the corresponding developmental age.