Table 1.
Details of species-specific primers developed for N. lugens and S. furcifera
| Species | Primer name | Sequence (5′–3′) | bp | Tm(°C) | GC | Amplicon size (bp) | Specificity at Ta (°C) | |||
|---|---|---|---|---|---|---|---|---|---|---|
| 46 | 50 | 55 | 64 | |||||||
| N. lugens | SNL4F | TTCTGACTTTTACCCCCATCTTTA | 24 | 57.59 | 37.50 | 307 | ✗ | ✗ | ✗ | ✓ |
| SNL4R | CAGCTAGAACAGGAAGGGATAGAA | 24 | 61.01 | 45.83 | ✗ | ✗ | ✗ | ✓ | ||
| SNBPH1F | TATTTGGTCAGGATTTATAGGA | 22 | 52.80 | 31.82 | 430 | ✗ | ✗ | ✗ | ✗ | |
| SNBPH1R | TGAAATAAAATTAATTGCAC | 20 | 45.00 | 20.00 | ✗ | ✗ | ✗ | ✗ | ||
| SNBPH2F | GTAGTATAATTATCCGATCAGA | 22 | 52.80 | 31.82 | 491 | ✗ | ✗ | ✗ | ✗ | |
| SNBPH2R | AAGGAGTAAAATAGCTGTAAT | 21 | 50.06 | 28.57 | ✗ | ✗ | ✗ | ✗ | ||
| SNLF3F | TTCTGACTTTTACCCCCATCTTTA | 24 | 57.59 | 37.50 | 302 | ✗ | ✗ | ✗ | ✗ | |
| SNLR3R | AGAACAGGAAGGGATAGAAGGAGT | 24 | 61.01 | 45.83 | ✗ | ✗ | ✗ | ✗ | ||
| S. furcifera | SNF2F | TCGATCTGAACTAACCCAACCT | 22 | 58.39 | 45.45 | 349 | ✗ | ✗ | ✗ | ✓ |
| SNF2R | GGCAATGTGGAGGGAGAAAAT | 21 | 57.87 | 47.62 | ✗ | ✗ | ✗ | ✓ | ||
| SNWBPH1F | GGTATGATCCGGACTAATTGGT | 22 | 58.39 | 45.45 | 510 | ✗ | ✗ | ✗ | ✗ | |
| SNWBPH1R | ACTGCGGTAATTAAAACTGAT | 21 | 52.01 | 33.33 | ✗ | ✗ | ✗ | ✗ | ||
| SNWBPH2F | AAGAATTTTAATTCGATCTGA | 21 | 48.11 | 23.81 | 401 | ✗ | ✗ | ✗ | ✗ | |
| SNWBPH2R | TTGAAATGAAATTGATAGCTCC | 22 | 52.80 | 31.82 | ✗ | ✗ | ✗ | ✗ | ||
Tm melting temperature, Ta annealing temperature, GC Guanine-Cytosine content