Table 2.
Synthetic positive controls (gBlocks®) for assay validation; changes were introduced in the WT sequence (GenBank® accession number: LS997562.1
| gBlock® | Length (bp) | Species | Codon 167 | Codon 198 | Codon 200 |
|---|---|---|---|---|---|
| 1 | 497 | H. contortus | TTC (s) | GAA (s) | TAC (r) |
| 2 | 497 | H. contortus | TAC (r) | GAA (s) | GAA (s) |
| 3 | 374 | H. contortus | TTC (s) | GCA (r) | GAA (s) |
| 4 | 374 | H. contortus | TTC (s) | TTA (r) | GAA (s) |
| 5 | 357 | T. circumcincta | TAC (r) | GAA (r) | TAC (r) |
| 6 | 357 | T. circumcincta | TTC (s) | GCA (s) | TTC (s) |
| 7 | 357 | T. circumcincta | TTC (s) | TTA (r) | TTC (s) |
| 8 | 514 | T. colubriformis | TAC (r) | GAA (r) | TAC (r) |
| 9 | 514 | T. colubriformis | TTC (s) | GCA (s) | TTC (s) |
s: susceptible, r: resistant.