Table 5.
Single nucleotide polymorphism frequencies for Haemonchus contortus, Italy (Bosco et al., 2020) (BT = before treatment, no post treatment data were available; <1% = below background threshold; *adjusted detection limit due to low background; excl. = excluded as not enough copies of the targeted Haemonchus-DNA were amplified; ND = no data [not analysed or no material available]; bold numbers = resistance alleles detected), 95% confidence intervals and relative uncertainty are shown in the supplementary file 4.
| Farm | Drug | N larvae in pooled samples BT/PT | % of Haemonchus larvae BT/PT | % F200Y BT | % F167Y BT | % E198A BT | % egg count reduction |
|---|---|---|---|---|---|---|---|
| 1 | ML | 43067/2600 | 78/7 | <1 | <1 | <2* | 97.9 (97.1–98.5) S |
| 2 | 33333/33 | 77/24 | <1 | <1 | <1 | 99.9 (9.4–100) S | |
| 3 | 8733/83 | 67/50 | 7 | <1 | <6* | 99.1 (98.3–99.6) S | |
| 4 | 4700/17 | 67/0 | <3* | excl. | excl. | 99.7 (99.3–99.9) S | |
| 5 | 11533/683 | 73/73 | <4* | <4* | 4* | 97.8 (97.2–98.3) S | |
| 6 | 55133/2050 | 93/11 | 5 | <1 | <1 | 97.8 (97.4–98.2) S | |
| 7 | 2250/0 | 28/ND | excl. | excl. | excl. | 99.5 (97.3–100) S | |
| 8 | 5267/0 | 65/ND | <2* | <2* | <2* | 99.8 (99.2–100) S | |
| 9 | 12533/0 | 83/ND | <1 | <1 | <1 | 100 (99.5–100) S | |
| 10 | 4000/0 | 38/ND | excl. | <7* | <6* | 96.7 (95.8–97.5) S | |
| 1 | BZ | 14000/300 | 79/29 | <1 | <1 | <1 | 73.7 (64.3–81.0) R |
| 2 | 17800/367 | 78/6 | <1 | <1 | <1 | 92.6 (89.9–94.7) SR | |
| 3 | 28133/483 | 72/59 | 8 | <1 | <1 | 97.0 (96.2–97.7) S | |
| 4 | 8133/17 | 68/82 | <1 | <1 | <1 | 100 (99.6–100) S | |
| 5 | 1400/50 | 21/11 | excl. | excl. | excl. | 99.6 (98.9–99.9) S | |
| 6 | 28800/533 | 32/30 | 6 | <4* | <4* | 99.6 (99.5–99.8) S | |
| 7 | 5267/0 | 37/ND | excl. | <13* | <3* | 99.8 (98.8–100) S | |
| 8 | 6533/0 | 61/ND | <2* | <1 | <2* | 100 (99.3–100) S | |
| 9 | 7800/0 | 81/ND | 84 | <8* | <8* | 97.7 (96.6–98.5) S | |
| 10 | 2333/0 | 29/ND | excl. | <6* | <6* | 100 (99.6–100) S |