Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 11;14:4115. doi: 10.1038/s41467-023-39447-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — A Schematics of the computational strategy for spatial transfer of modalities followed by gene-structure correlation analysis. Unmeasured genes in MERFISH are imputed from SmartSeq2 data. MERFISH and EM data from adjacent sections are registered to common coordinate space. Gene expression values as well as structural feature values are then transferred between neighboring cells using distance-weighted averaging. B Rank plots of Spearman correlation between genes and selected structural features (top). Barplots on the bottom show enriched Gene Ontology Cellular Component terms among the top 200 positively (shades of red) or negatively (shades of green) correlating genes with respective structural feature. One-sided hypergeometric test, nominal p-value. C Heatmaps showing correlation between selected genes and structural features in microglia (red-green palette), average values of spatially transferred modality in ST and EM clusters (red-blue palette), and average values of directly measured modality in ST and EM clusters (orange-purple palette). D Heatmap of multiscale Earth Mover’s distances (EMD) between feature-based structural clusters and ST-derived clusters. Z-scored values per structural cluster (row scaling) as well as per ST cluster (column scaling) are shown. A was created with elements from BioRender.com.