Table 1:
Macrophage Flow Cytometry Antibodies
| Antibody | Antibody Clone | Antibody Source | Fluorochrome | Marker Type |
|---|---|---|---|---|
| CD3 | OKT3 | Biolegend | BV 421 | Lymphocytes |
| CD15 | W6D3 | Biolegend | BV 421 | Neutrophils |
| Live/Death | N/A | Thermo Scientific | Aqua | Live Cells |
| CD86 | IT2.2 | Thermo Scientific | SB600 | M1 Macrophages |
| CD14 | M5E2 | Biolegend | BV 650 | Monocyte Subtypes |
| CD45 | HI30 | Thermo Scientific | Qdot800 | Leukocytes |
| CD68 | Y1/82A | Biolegend | FITC | Macrophages |
| Siglec 8 | 7C9 | Biolegend | PerCP-Cy5.5 | Eosinophils |
| S100A | NJ-4F3-D1 | Biolegend | PE | Pro-inflammatory Marker |
| CD169 | 7–239 | Biolegend | PE/CYN7 | Tissue-resident macrophages |
| CD206 | 15–2 | Biolegend | APC | M2 Macrophages |
| CD16 | B73.1 | Biolegend | AlexaFluor 700 | Monocyte Subtypes |
| CD11B | M1/70 | Thermo Scientific | APCefluor780 | Macrophage |