Fig. 2.

ESC-EVs rejuvenate the transcriptome profile of senescent MEFs. (A) A scatter plot illustrating differentially regulated gene expression from RNA-Seq analysis between the Ctrl (P7 MEFs) and ESC-EVs treated P7 MEFs. Upregulated and downregulated genes are shown in red and blue, respectively. (p < 0.05 and FC ≥ 2 or FC ≤ 0.5). (B) Heat map of representative cell aging (above) and cell proliferation-related genes (bottom). (C–F) GSEA analysis identifies enrichment scores for modules of cell aging (C), p53 pathway (D), PI3K-AKT pathway (E) and cell proliferation (F). ES, enrichment score. NES, normalized enrichment score.