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. 2023 Jul 1;29:85–97. doi: 10.1016/j.bioactmat.2023.06.011

Fig. 3.

Fig. 3

miRNA landscape of ESC-EVs. (A) Hierarchical clustering of the miRNA-Seq analysis results shows differentially expressed miRNAs (p < 0.05) between the ESC-EVs and MEF-EVs. (B) A volcano plot illustrating differential miRNA expression from miRNA-Seq analysis between the ESC-EVs and MEF-EVs. Up-regulated and down-regulated miRNAs are shown in red and blue, respectively. (p < 0.05 and FC ≥ 2 or FC ≤ 0.5). (C) KEGG pathway analysis of the target genes of significantly enriched miRNAs (p < 0.05 and FC ≥ 2) in ESC-EVs vs MEF-EVs. (D) Western blot analysis of senescence-related proteins after overexpression of 6 candidate miRNAs in the senescent MEFs. (E) RT-qPCR analysis for the expression of senescence-related genes after overexpression of 6 candidate miRNAs in the senescent MEFs. (F) SA-β-gal staining of senescent MEFs overexpressing of 6 candidate miRNAs. Scale bar represents 100 μm. (G) RT-qPCR analysis for the expression of miR-15b-5p and miR-290a-5p in P3 and P7 MEFs. Data are presented as mean ± SEM. n = 3. Student's t-test: ns, not significance; *p < 0.05 and **p < 0.01.