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. 2023 Jul 1;29:85–97. doi: 10.1016/j.bioactmat.2023.06.011

Fig. 4.

Fig. 4

miR-15b-5p and miR-290a-5p rejuvenate aged cells. (A) Western blot analysis of p21 and p53 in P7 MEFs overexpressing of miR-15b-5p or miR-290a-5p. (B) Grayscale statistical analysis of p21 and p53 relative to H3. (C) Immunofluorescence analysis of p21 and p53 in P7 MEFs overexpressing of miR-15b-5p or miR-290a-5p. Scale bar represents 100 μm. (D) Quantification of the percentage of p21 and p53 positive MEFs respectively. (E) Immunofluorescence analysis of Ki67 and γ-H2AX in P7 MEFs overexpressing of miR-15b-5p or miR-290a-5p. Scale bar represents 100 μm. (F) Quantification of the percentage of Ki67 and γ-H2AX positive MEFs respectively. (G) Cell cycle of P3 MEFs and P7 MEFs overexpressing of miR-15b-5p or miR-290a-5p was depicted by G0/G1, S and G2/M phases. (H) Quantification of the ratio of ROS-positive cells in each group to the negative control group. Data are presented as mean ± SEM. n = 3. Student's t-test: ns, not significance; *p < 0.05 and **p < 0.01.