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. 2023 Jun 28;11:1219883. doi: 10.3389/fchem.2023.1219883

TABLE 2.

Trans-activating CRISPR RNA (crRNA) and repair template (single-stranded oligodeoxynucleotide or ssODN) sequences used to generate stable HSP90α/β-HiBit tagged knock-in cells. The left and the right homology arms for the respective target genes in the ssODN sequence are represented in upper case, separated by the HiBit-tag, represented in lower case.

Construct Sequence
crRNA HSP90AA1 (HSP90α) 5’-AGU​AGA​CUA​AUC​UCU​GGC​UGG​UUU​UAG​AGC​UAU​GCU-3’
crRNA HSP90AB1 (HSP90β) 5’-UCG​CAU​GGA​AGA​AGU​CGA​UUG​UUU​UAG​AGC​UAU​GCU-3’
tracrRNA Universal 67mer
ssODN HSP90AA1 5’- TGC​CAC​CCC​TTG​AAG​GAG​ATG​ACG​ACA​CAT​CAC​GCA​TGG​AAG​AAG​TAG​ACg​tga​gcg​gct​ggc​ggc​tgt​tca​aga​aga​tta​gcT​AAT​CTC​TGG​CTG​AGG​GAT​GAC​TTA​CCT​GTT​CAG​TAC​TCT​ACA​ATT​CCT​C-3’
ssODN HSP90AB1 5’- TCC​CCC​CTC​TCG​AGG​GCG​ATG​AGG​ATG​CGT​CTC​GCA​TGG​AAG​AAG​TCG​ATg​tga​gcg​gct​ggc​ggc​tgt​tca​aga​aga​tta​gcT​AAG​TTA​GAA​GTT​CAT​AGT​TGA​AAA​ACT​TGT​GCC​CTT​GTA​TAG​TGT​CCC​C-3’