REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Insulin (1:500)	Dako	Cat# A-0546
Glucagon (1:2000)	Sigma	Cat# G2654
Somatostatin (1:200)	Millipore	Cat# MAB354
AlexaFluor® 488 goat anti-guineapig (1:500)	Invitrogen	Cat# A11073
AlexaFluor® 546 goat anti-rat (1:500)	Thermo Fisher	Cat# A11077
AlexaFluor® 647 goat anti-mouse (1:500)	Thermo Fisher	Cat# A21235
Biological samples
Human pancreas slices	nPOD	www.jdrfnpod.org
Human isolated islets	Prodo Laboratories
Chemicals, peptides, and recombinant proteins
Alanine	Sigma	Cat# A7627
Aprotinin	Sigma	Cat# A1153
Arginine	Sigma	Cat# A5006
Bovine berum albumin	Sigma	Cat# 9048-46-8
Calbryte 520 AM	AAT Bioquest	Cat# 20651
Calcium chloride	Sigma	Cat# C4901
Epinephrine	Sigma	Cat# E4375
Fluo4-AM	Invitrogen	Cat# F14201
Glutamic acid	Tocris	Cat# 0218
Glutamine	Sigma	Cat# G3126
Hepes	Sigma	Cat# 7365-45-9
Kainic acid	Tocris	Cat# 0222
Low melting point agarose	Sigma	Cat# A9414
Magnesium chloride	Millipore	Cat# M8266
Potassium chloride	Sigma	Cat# P9541
Sodium chloride	Millipore	Cat# 567440
Critical commercial assays
Insulin ELISA (human)	Mercodia	Cat# 10-1113-01
Glucagon ELISA (human kit)	Mercodia	Cat# 10-1271-01
Glucagon ELISA 10 μL (for animal samples)	Mercodia	Cat# 10-1281-01
Experimental models: Organisms/strains
C57Bl6J (mouse, male, 8–16 weeks)	The Jackson Laboratory	Stock #000664
Gcg/tm 1.1(icre)Gkg/J (mouse, female, 8–16 weeks)	The Jackson Laboratory	Stock #030663
B6.Cg-Gt(ROSA)26Sor(tm38(CAG-GCaMP3)HZE) (mouse, male, 8–16 weeks)	The Jackson Laboratory	Stock #029043
Software and algorithms
Prism 6	GraphPad	Prism 9
Fiji	Schindelin et al.2	https://fiji.sc/
Matlab	MathWorks	R2019a
Other
Semi-automatic vibratome VT1200S,	Leica	Cat#14048142066
SP5 upright laser-scanning confocal microscope	Leica
Perifusion system with automated tray handling	Biorep Technologies	PERI4-02-230-FA
Pancreas slice chamber	Biorep Technologies	PERI-PSC-001
Pancreas slice chamber extender kit	Biorep Technologies	PERI-PSC-EXT
Pancreas slice chamber perforated plate	Biorep Technologies	PERI-PSC-PP