Table 3.
Differentially expressed mitochondrial proteins upon hypovirus infection.
| Protein IDa | Protein name | Average change (EP713/EP155)b | Unique PepCountc | Cover percentd | MWe | pIf |
|---|---|---|---|---|---|---|
| Carbohydrate metabolism | ||||||
| 266329 | 2-oxoisovalerate dehydrogenase subunit beta | 2.0 | 1 | 2.1 | 41 | 6.7 |
| 346917 | Citrate synthase | 1.8 | 5 | 11.8 | 52 | 7.7 |
| 100278 | Oligosaccharyl transferase STT3 subunit | 1.8 | 1 | 2.4 | 83 | 8.2 |
| 342893 | Dolichyl-diphosphooligosaccharide-protein (Oligosaccharyltransferase, gamma subunit) | 1.6 | 1 | 2.4 | 37 | 8.7 |
| 323583 | 6-phosphogluconate dehydrogenase, decarboxylase | 0.7 | 9 | 17.1 | 54 | 5.9 |
| 293608 | Alpha-1,3-glucan synthase | 0.6 | 1 | 0.5 | 263 | 5.8 |
| 104818 | Glycoside hydrolase family 72 protein | 0.6 | 1 | 2.6 | 57 | 4.3 |
| 101684 | Glyceraldehyde-3-phosphate dehydrogenase | 0.5 | 13 | 36.5 | 36 | 7.0 |
| Amino acid metabolism | ||||||
| 277618 | Proline dehydrogenase | 3.0 | 4 | 9.4 | 48 | 8.4 |
| 347121 | Cyanide hydratase | 2.7 | 3 | 8.1 | 40 | 5.5 |
| 98901 | Delta-1-pyrroline-5-carboxylate dehydrogenase | 2.2 | 20 | 39.3 | 63 | 8.3 |
| 297033 | Oxidoreductase (Kynurenine 3-monooxygenase) | 1.8 | 2 | 7.2 | 49 | 6.7 |
| 355226 | S-adenosylmethionine synthase | 0.7 | 4 | 12.1 | 43 | 6.3 |
| 106743 | Carbamoyl-phosphate synthase subunit | 0.6 | 5 | 12.9 | 50 | 7.2 |
| 99282 | Asparagine synthetase | 0.5 | 2 | 3.1 | 66 | 6.4 |
| 320111 | Carboxypeptidase y protein | 0.4 | 1 | 1.5 | 61 | 4.9 |
| Lipid metabolism | ||||||
| 344593 | PAP2-domain-containing protein | 2.0 | 2 | 6.3 | 37 | 8.5 |
| 280920 | Delta fatty acid desaturase protein | 0.6 | 1 | 2.4 | 55 | 8.0 |
| 275917 | Acyl-coenzyme A oxidase | 0.5 | 2 | 4.3 | 78 | 8.8 |
| 321389 | 3-ketoacyl-thiolase protein | 0.5 | 1 | 2.2 | 44 | 6.3 |
| 322936 | Cyclopentanol dehydrogenase | 0.3 | 2 | 6.7 | 30 | 6.4 |
| Energy metabolism | ||||||
| 337351 | Cytochrome c peroxidase | 2.2 | 6 | 23.5 | 42 | 7.9 |
| 358627 | Short-chain dehydrogenase | 2.2 | 5 | 16.2 | 38 | 8.9 |
| 348284 | Cytochrome P450 | 1.9 | 3 | 3.5 | 106 | 7.2 |
| 248835 | Monooxygenase FAD-binding protein | 1.8 | 1 | 2.3 | 48 | 6.4 |
| 343596 | Oxidoreductase | 1.7 | 1 | 2.3 | 46 | 8.5 |
| 323634 | Thioredoxin protein | 1.6 | 2 | 6.8 | 37 | 4.9 |
| 285853 | Formate dehydrogenase | 1.5 | 9 | 24.3 | 49 | 8.5 |
| 339855 | Oxidoreductase domain containing protein | 1.5 | 3 | 8.7 | 46 | 7.9 |
| 322591 | Flavocytochrome c | 0.6 | 13 | 20.7 | 68 | 6.7 |
| 295721 | NADH:flavin oxidoreductase NADH oxidase | 0.6 | 2 | 4.9 | 44 | 5.9 |
| 357133 | NADP-dependent leukotriene B4 12-hydroxydehyd | 0.5 | 2 | 7.1 | 31 | 6.9 |
| 355099 | Inorganic pyrophosphatase | 0.5 | 5 | 18.4 | 33 | 5.6 |
| 357360 | Mitochondrial peroxiredoxin prx1 | 0.5 | 6 | 22.7 | 26 | 6.2 |
| 253099 | NADH:flavin oxidoreductase/NADH oxidase | 0.3 | 3 | 8.7 | 50 | 6.4 |
| Transport | ||||||
| 356670 | Mitochondrial phosphate carrier protein | 2.7 | 3 | 6.8 | 42 | 9.2 |
| 248376 | Integral membrane protein | 1.9 | 1 | 5.4 | 19 | 9.1 |
| 102464 | ADP-ribosylation factor | 1.7 | 2 | 9.8 | 21 | 6.6 |
| 355657 | Rab GDP-dissociation inhibitor | 1.7 | 5 | 11.7 | 51 | 5.9 |
| 74450 | Mitochondrial Rho GTPase | 1.5 | 2 | 3.2 | 70 | 6.0 |
| 322230 | Methyltransferase | 1.5 | 2 | 12.1 | 30 | 4.9 |
| 96221 | BAR-domain-containing protein | 0.6 | 1 | 4.6 | 30 | 6.4 |
| 273042 | RHO3 protein | 0.6 | 1 | 4.3 | 23 | 4.8 |
| 67026 | GNAT family acetyltransferase | 0.4 | 1 | 5.5 | 24 | 6.6 |
| 358399 | N-acetyltransferase-like protein | 0.4 | 2 | 8.8 | 31 | 5.9 |
| 245698 | 3-oxoacyl-[acyl-carrier-protein] reductase | 0.3 | 2 | 2.2 | 83 | 8.5 |
| 102797 | Serine-type endopeptidase-like protein | 0.2 | 3 | 6.5 | 55 | 5.6 |
| Signal transduction | ||||||
| 285804 | Alkaline phosphatase family protein | 2.0 | 2 | 3.3 | 68 | 5.8 |
| 281836 | Telomere silencing protein | 0.7 | 1 | 1.2 | 98 | 6.7 |
| 66486 | Serine/threonine-protein kinase 12 | 0.6 | 1 | 1.6 | 104 | 9.1 |
| 336942 | Acyl-coenzyme A oxidase | 0.5 | 1 | 1.2 | 83 | 6.7 |
| 108039 | Serine threonine kinase irei protein | 0.4 | 1 | 0.7 | 131 | 6.3 |
| Mitochondrial morphology | ||||||
| 100977 | Importin subunit alpha | 1.5 | 8 | 14.6 | 60 | 5.1 |
| 344061 | VTS1 protein | 0.7 | 1 | 1.3 | 65 | 8.6 |
| 320360 | 60S ribosomal protein L8 | 0.6 | 1 | 3.5 | 28 | 11.0 |
| 292762 | RNA interference and gene silencing protein | 0.4 | 2 | 2.3 | 103 | 9.2 |
| Others | ||||||
| 323083 | Lea domain-containing protein | 1.6 | 1 | 8.0 | 13 | 9.8 |
| 292269 | Hrf1 domain-containing protein | 1.5 | 1 | 3.8 | 36 | 9.7 |
| 102543 | Sphingolipid long chain base-responsive protein | 0.6 | 4 | 11.2 | 40 | 5.6 |
| 104580 | Transcription factor | 0.6 | 1 | 4.2 | 36 | 8.7 |
| Unknown | ||||||
| 350456 | Uncharacterized protein | 1.7 | 1 | 1.5 | 43 | 5.2 |
| 337183 | Uncharacterized protein | 2.7 | 3 | 6.8 | 45 | 9.1 |
| 321541 | Uncharacterized protein | 2.0 | 4 | 37.2 | 13 | 11.3 |
| 356299 | Uncharacterized protein | 11.3 | 1 | 15.1 | 11 | 11.3 |
| 330389 | Uncharacterized protein | 7.5 | 2 | 6.0 | 60 | 6.8 |
| 258562 | Uncharacterized protein | 1.9 | 6 | 28.1 | 31 | 9.9 |
| 340397 | Uncharacterized protein | 0.6 | 1 | 2.4 | 56 | 6.6 |
| 247407 | Uncharacterized protein | 0.6 | 1 | 8.2 | 10 | 5.1 |
| 346932 | Uncharacterized protein | 0.6 | 1 | 2.7 | 53 | 8.0 |
The list of up/downregulated mitochondrial protein upon hypovirus infection by HPLC-ESI-OrbiTrap MSMS.
aAccession number from Cryphonectria parasitica database v2.0.
bHypovirus-free strain EP155 was set as reference. The average change (EP713/EP155) were from three independent experimental data.
cThe total number of unique matched peptides to the identified protein.
dThe cover percentage of matched peptides to the protein sequence.
eTheoretical molecular weight (kDa).
fTheoretical pI.