Table 5.
Optimum singleplex real-time PCR reaction conditions.
| Pathogens | Final concentration (μmol/μL) | |
|---|---|---|
| Forward and reverse primer | TaqMan probe | |
| Cart A | 0.5 | 0.3 |
| Cart B | 0.6 | 0.3 |
| Cart E | 0.6 | 0.6 |
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