Table 7.
Repeatability of the multiplex real-time PCR assay.
| Standard plasmid | Concentration of template (copies/μL) | Intra-coefficient of variation | Inter-coefficient of variation | ||
|---|---|---|---|---|---|
| X ±SD | CV (%) | X ±SD | CV (%) | ||
| pMD-Cart A | 107 | 17.250 ± 0.061 | 0.35 | 17.547 ± 0.040 | 0.23 |
| 105 | 23.437 ± 0.100 | 0.43 | 23.510 ± 0.286 | 1.21 | |
| 103 | 31.810 ± 0.060 | 0.19 | 31.337 ± 0.202 | 0.65 | |
| pMD-Cart B | 107 | 17.370 ± 0.262 | 1.51 | 17.530 ± 0.040 | 0.23 |
| 105 | 24.230 ± 0.270 | 1.11 | 24.787 ± 0.176 | 0.71 | |
| 103 | 31.080 ± 0.036 | 0.12 | 31.677 ± 0.176 | 0.12 | |
| pMD-Cart E | 107 | 17.660 ± 0.191 | 1.08 | 17.477 ± 0.153 | 0.87 |
| 105 | 23.277 ± 0.230 | 0.99 | 23.467 ± 0.233 | 0.99 | |
| 103 | 31.570 ± 0.303 | 0.96 | 31.580 ± 0.384 | 1.22 | |