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. 2023 Jun 26;9(7):e17657. doi: 10.1016/j.heliyon.2023.e17657

Table 2.

Overview of recent studies reporting probable modes of action of phenolic compounds on AHL-mediated QS in bacteria.

Phenolic compound (potential QSI) MIC Anti-QS effect (phenotypes) Mechanism and Method References
Resveratrol >1000 μg/mL against P. aeruginosa PAO1 and clinical isolates. Inhibition of biofilm formation and enhancement of the effects of aminoglycoside antibiotics. Lowering expression of lasI and rhlI, analyzed by qRT-PCR. [121]
Curcumin and resveratrol >100 μΜ for C. violaceum ATCC 12472 and 026. Inhibition of violacein, biofilm formation and swarming motility. In silico study (docking) with CviR QS regulator of C. violaceum. [122]
Gingerol >50 μg/mL for P. aeruginosa AM26. Reduced biofilm formation, exopolysaccarides (EPS), pyocyanin and rhamnolipids. In silico studies - docking and molecular dynamics - with LasR, RhlR, PhzR. [123]
Phloretin, chrysin, quercetin, baicalein. >100 μΜ for P. aeruginosa PA14. Suppression of virulence factors production in P. aeruginosa. Allosteric inhibition of LasR/RhlR. [95]
Methyl gallate 512 μg/mL for P. aeruginosa PAO1. Reduction on biofilm formation, EPS, swarming motility, and virulence factors in P. aeruginosa. Reduced expression of lasI, lasR, rhlI, and rhlR. [70]
Vanillic acid >250 μg/mL for S. marcescens MG1 and clinical isolate; >500 μg/mL for S. marcescens ATCC 14756. Reduction of biofilm formation and expression of virulence factors in S. marcescens. Affecting S-layer, flagellin and fatty acid biosynthesis proteins. [107]
Rosmarinic acid >100 μΜ for P. aeruginosa PAO1. Induced the expression of 128 genes, many implicated in QS; and seven sRNAs encoded in regions close to QS-induced genes. In silico study, RNA-Seq and qRT-PCR analysis. [124]
Naringenin >1 μM for E. coli LasR-based biosensor strain. Inhibited QS-regulated virulence factors in P. aeruginosa PAO1. Reduced expression of QS-regulated genes by directly binding to LasR, competing with its cognate activator (3OC12-HSL). [125]
Chlorogenic acid 5.12 mg/mL for P. aeruginosa PAO1 and C. violaceum. Inhibited biofilm formation, swarming and violacein production in C. violaceum; inhibited virulence factors in P. aeruginosa PAO1 and attenuated C. elegans killing by P. aeruginosa. In silico study (LasR, RhlR, PqsR); reduced expression of QS-related genes (lasI, lasR, rhlI, rhlR, pqsA, pqsR, cviI). [126]