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. Author manuscript; available in PMC: 2023 Jul 12.
Published in final edited form as: ACS Appl Mater Interfaces. 2017 Oct 31;9(45):39185–39196. doi: 10.1021/acsami.7b12029

Figure 2. Time course of nHAp stimulated osteopontin and repressed alkaline phosphatase gene expression;

Figure 2.

BMSCs were cultured in; growth medium (GM), GM with ascorbic acid (AA) added at the same time as 25 μg/ml of nHAp (AA-same) or 24hrs before nHAp (AA-pre) and RNA was analyzed by qRT-PCR for (A) osteopontin (Opn) or (B) Alkaline phosphatase (Alp) expression levels. Right panels; an expanded graph of early time points calculated as fold change from untreated control. **p<0.005 and ***p<0.0005 (one-way ANOVA).