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. Author manuscript; available in PMC: 2023 Jul 12.
Published in final edited form as: ACS Appl Mater Interfaces. 2017 Oct 31;9(45):39185–39196. doi: 10.1021/acsami.7b12029

Figure 4. nHAp stimulates phosphorylation of Erk1/2.

Figure 4.

(A) BMSCs were treated with nHAp (25 μg/ml) for indicated times and analyzed by Western blotting or phospho-Erk1/2 (pErk1/2) or total Erk1/2. (B) Cells were serum starved and treated with nHAp and samples analyzed by Western blotting as indicated; p-phosphorylated form. (C) Cells were pre-treated with inhibitors for Erk1/2 (iErk1/2), Jnk (iJnk), and p38 (ip38) and the resulting lysate was analyzed by Western blotting and probed as indicated. (D) Cells were pretreated with Erk inhibitor for 24 hours and samples analyzed by qRT-PCR for osteopontin expression. $$p<0.005 compared to untreated control; **p<0.005 compared to the nHAp treated (Student’s t-test).