Table 2.
Batch‐to‐batch analysis of physicochemical properties, lipids, and liposoluble compounds of the NF
| Parameter (unit) | Batch number | Analytical methods | ||||
|---|---|---|---|---|---|---|
| #6 | #7 | #8 | #9 | #10 | ||
| Peroxide value (meqO 2 .kg − 1 ) | 0.2 | 0.3 | 0.1 | 0.1 | 0.1 | Potentiometry (NF EN ISO 27107) |
| Lipids (Relative % w/w of fat) | ||||||
| Triglycerides | 82 | 81 | 81.7 | 85.7 | 73.4 | Gas chromatography (internal) |
| Diglycerides | 3.5 | 4.5 | 4.3 | 2.8 | 3 | Gas chromatography (internal) |
| Monoglycerides | 1.6 | 1.5 | 1.2 | 1.2 | 1.3 | Gas chromatography (internal) |
| Total sterols | 0.3 | 1.3 | 0.8 | 0.5 | 0.8 | NF EN ISO 12228_part 1 |
| Cholesterol | < 0.1 | 0.1 | 0.4 | < 0.1 | < 0.1 | Gas chromatography (internal) |
| Unidentified | 0.6 | 0.9 | < 0.1 | < 0.1 | < 0.1 | Calculation |
| Free fatty acids (FFAs) | 12 | 10.6 | 11.7 | 9.8 | 21.5 | Gas chromatography (internal) |
| Total phospholipids (g/100 g of NF) | 5.2 | 2.26 | 5.04 | 4.19 | 3.96 | HPLC‐ELSD (internal) |
| Total fucoxanthin (% w/w of NF) | 2.06 | 2.05 | 2.01 | 2.11 | 2.09 | HPLC‐DAD (internal) |
| Chlorophylls and derivatives | ||||||
| Chlorophyll C (g equivalent chlorophyll A /100 g of NF) | 2.93 | 3.73 | 3.52 | 4.15 | 3.37 | HPLC‐DAD (internal) |
| Pheophorbide A (g equivalent pheophorbide A /100 g of NF) | 1.88 | 1.59 | 3.35 | 2.01 | 2.15 | HPLC‐DAD (internal) |
| Pheophytin A (g equivalent chlorophyll A /100 g of NF) | ND (a) | 0.56 | 0.671 | 0.36 | 0.49 | HPLC‐DAD (internal) |
| Σ Chlorophyll B (g equivalent chlorophyll A /100 g of NF) | ND (a) | ND (a) | ND (a) | ND (a) | ND (a) | HPLC‐DAD (internal) |
| Σ Chlorophyll A (g equivalent chlorophyll A /100 g of NF) | ND (a) | 0.651 | 0.031 | 0.169 | 0.122 | HPLC‐DAD (internal) |
NF: novel food; ND: not detected; HPLC‐DAD: high‐performance liquid chromatography–diode‐array detection; EN: Europaische Norm (European Standard); ISO: International Organization for Standardization; FFA: free fatty acid; HPLC‐ELSD: high‐performance liquid chromatography–evaporative light scattering detection.
(a)
The applicant informed EFSA that the limits of quantification were not determined because the contents found in the samples were well above the minimum quantities injected during calibration.