Table 1.

Indole oxidation catalyzed by P450 BM3^T mutants in the presence of H₂O₂.

Mutant names	Mutations	TON [a]	Indigo : indirubin [b]
P450 BM3T1	A184N L188S	1226 ± 63	92:8
P450 BM3T2	A184N L188Y	1002 ± 46	92:8
P450 BM3T3	A184N L188F	883 ± 52	91:9
P450 BM3T4	A184N L188H	810 ± 43	90:10
P450 BM3T5	A180I L181F	732 ± 52	94:6
P450 BM3T6	A180R L181H	523 ± 32	91:9
P450 BM3T7	A184N L188N	407 ± 26	93:7
P450 BM3T8	A180I L181Y	321 ± 22	95:5
P450 BM3T9	A82F	303 ± 26	94:6
P450 BM3T10	A180F L181V	240 ± 18	95:5

^aTON: Turnover numbers (TON) were determined based on the decreased amounts of indole from triplicated reactions in 30 min.

^bIndigo : indirubin: the ratios between indigo and indirubin were quantitatively determined by monitoring their maximum absorption peaks using HPLC. Reaction conditions: 0.1 μM P450 BM3^T enzyme, 1 mM indole, and 60 mM H₂O₂ in 100 mM potassium phosphate buffer (pH 8.0), at 30 °C.