Table 2.

P450 BM3^T1 as the biocatalyst in the oxidative transformation of indole and its derivatives.

Substrates	TON a	symmetric : asymmetric b
1	1226 ± 63	92:8
2	1206 ± 33	98:2
3	2263 ± 29	92:8
4	1557 ± 42	59:41
5	2142 ± 43	68:32
6	1535 ± 22	98:2
7	1970 ± 17	91:9
8	2014 ± 42	86:14
9	2291 ± 55	70:30
10	1677 ± 20	99:1
11	1495 ± 39	90:10
12	1978 ± 29	41:59
13	2191 ± 16	90:10
14	1761 ± 23	89:11
15	1953 ± 19	88:12
16	2082 ± 34	93:7
17	1760 ± 22	99:1
18	1073 ± 16	80:20
19	2331 ± 37	52:48
20	1433 ± 21	92:8
21	1810 ± 63	90:10
23	1783 ± 40	61:39
24	1697 ± 18	76:24
26	2061 ± 22	91:9
27	1679 ± 19	72:28
28	1767 ± 35	70:30
29	1086 ± 10	93:7
30	1663 ± 15	98:2
31	1292 ± 23	92:8
32	1107 ± 15	99:1
34	1182 ± 37	38:62
35	1713 ± 23	87:13
37	965 ± 14	96:4
39	2036 ± 24	85:15
41	1756 ± 26	92:8

^aTONs were calculated by averaging at least three independent experiments. General reaction condition: 0.1 μM P450-BM3^T1 enzyme, 1 mM substrate, and 60 mM H₂O₂ in 100 mM phosphate buffer (pH 8.0), 30 °C. The reactions were performed for 30 min in order to calculate TONs.

^bsymmetrical : asymmetric: the ratios of indigo and indirubin derivetives were determined by monitoring their maximum absorption peaks using HPLC.