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. 2023 Jun 15;9(7):4415–4430. doi: 10.1021/acsbiomaterials.3c00346

Figure 6.

Figure 6

Cell layer maturation and functional analysis of iHep when cultured inside MINERVA 2.0. (a) Z-stack projection of immunofluorescence confocal microscopy images of iHep in the COLL–PEG gel in static (left) and perfused (right) conditions 7 days post maturation. Green = albumin and blue = Hoechst nuclear staining. Magnification: 10X. (b) Cytotoxicity at days 1 and 7 in perfused samples. (c) Metabolic activity. (d) Albumin production. (e) Urea synthesis. (f,g) mRNA relative expression of CYP3A4 and HNF4a in static and perfused samples 7 days post maturation. Mann–Whitney U test ns = p > 0.05, * = p < 0.05; ** = p < 0.01.