Fig. 4 |. m⁶A deposition on retrotransposon-derived RNAs.

a, Relative enrichment of m⁶A peaks (identified in biological replicate 1) in eight major families from three types of retrotransposon. The enrichment score was calculated as the log₂ ratio of the observed over expected peak numbers. b, Heat map plots showing the ratios of genomic copies/loci with >0 m⁶A signal score (MeRIP versus Input) and >0 expression value (RPKM), respectively (calculated using biological replicate 1), for representative retrotransposon subfamilies. c, m⁶A marking status, m⁶A signal and expression profiles across the loci of MTA, MERVL and L1Md_T. For MTA and MERVL, only the internal sequences were considered. The line plots above the heat maps show the percentages of loci with m⁶A+ (that is, overlap with m⁶A peaks, left), >0 m⁶A signal value (middle), >0 expression value (right), respectively. Color range of m⁶A signal: MTA, −4.9 to 7.2; MERVL, −0.7 to 3.9; L1Md_T, −1.1 to 1.8. Color range of expression (that is, averaged log₂(RPKM)): MTA, 0 to 12; MERVL, 0 to 8; L1Md_T, 0 to 2. d, m⁶A signal density pileups along the full-length MTA, MERVL and L1Md_T loci. The lower bar plots show the frequency (bin size 10 bp) of GGACU or RRACH motifs across all loci along the full-length structure.