Extended Data Table 1 |.

Summary of picoMeRIP-seq data

Developmental Stage	Biological Replicate	# of oocytes or embryos	IP/Inpt	picoMeRIP-seq read pairs
				Clean*	Aligned	Uniquely aligned	Used**
GV oocyte	1	49	Input	13,940,666	12,410,243	11,534,472	8,081,463
			IP	64,772,174	55,156,245	47,354,440	6,599,699
	2	49	Input	14,495,984	12,834,133	11,933,879	8,688,556
			IP	54,588,178	44,866,161	38,536,527	8,393,016
MII oocyte	1	100	Input	12,006,076	10,713,624	9,838,618	7,587,810
			IP	44,916,482	36,251,485	30,205,801	5,947,926
	2	110	Input	13,886,140	11,826,450	10,682,706	7,700,702
			IP	57,664,412	46,392,988	38,217,234	4,339,674
Zygote	1	100	Input	14,717,358	12,929,450	11,796,400	8,187,579
			IP	50,367,925	40,283,164	33,652,477	2,068,664
	2	100	Input	10,004,278	8,838,762	8,101,986	6,209,041
			IP	54,374,881	43,285,231	36,251,025	4,272,507
2-cell	1	100	Input	12,947,189	10,565,632	9,350,999	4,752,917
			IP	76,183,532	46,971,391	40,175,160	1,563,343
	2	100	Input	12,221,379	9,544,819	8,486,166	4,616,710
			IP	48,265,377	37,154,826	31,639,346	2,049,945
8-cell	1	100	Input	17,389,588	13,425,044	12,409,834	5,827,766
			IP	45,397,104	37,069,105	34,088,762	1,616,221
	2	51	Input	13,082,644	10,849,512	9,939,893	3,918,751
			IP	52,437,784	43,405,840	39,906,864	2,604,341
Blastocyst	1	100	Input	16,915,541	10,350,601	9,446,996	4,100,958
			IP	50,412,477	41,518,869	38,740,886	2,316,236
	2	100	Input	15,336,531	8,857,713	8,053,118	1,678,558
			IP	59,223,012	43,995,716	40,822,515	927,571

^*Read pairs after control (by FastQC) and adapter removal (by cutadapt)

^**Uniquely-aligned read pairs after removing PCR duplicates and ribosomal RNA-derived reads; and these reads were used for m6A peak calling by MACS2