Fig. 7.

JAK2/STAT3 pathway regulates ER stress and apoptosis through HAX1. A–D Western blotting and statistical analyses of BIP, CHOP, ATF6, ATF4, IRE1, p-IRE1, XBP-1s, BCL2, BAX, PUMA, NOXA, GADD45A, caspase-9, caspase-3, and β-actin proteins after AG490 and OE-HAX1 treatment in H/R cell model of BRL-3A (n = 3 for each group). p-IRE1 indicates phospho-IRE1, C-caspase indicates cleaved caspase, OE-HAX1 indicates overexpressed HAX1. E Caspase-3 activity detection (n = 6 for each group). F, G Flow cytometric analysis used for the detection of apoptosis after AG490 and OE-HAX1 treatment in H/R cell model of BRL-3A (n = 3 for each group). H, I Flow cytometric analysis used for the detection of mitochondrial membrane potential in H/R cell model of BRL-3A (n = 3 for each group). ^*p < 0.05 and ^**p < 0.01