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. 2023 Jul 11;11:113. doi: 10.1186/s40478-023-01610-0

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Validation of the study findings in an independent data set (GSE205331) [14] (a) Clustering the samples with a set of 10 010 array probes (variable probes from both experiments, occurring in both EPIC and HM450K arrays) with heatmap of scaled methylation M-values (b) Beta-values of probes with standard deviation of β-values above 0.1, split according to CpG relation to CGI (c) Difference in the methylation levels of cg12044599 CpG in PTPRCAP locus in chordoma C and I subtypes in samples from current study, validation data set and in metaclusters (d) Difference in the content of CD8⁺ lymphocytes in chordoma C and I subtypes from current of study group, validation data set and in metaclusters