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. 2023 Jun 28;14:1157397. doi: 10.3389/fimmu.2023.1157397

Figure 2.

Figure 2

PD-L1 is predominantly expressed in tumor areas characterized by high infiltration of TAMs and CD8+ T cells in primary PDAC tissues and corresponding liver metastases. First, quantitative marker distribution of PD-L1 (A), CD68 (D), CD163 (G) and CD8 (J) in primary tumors (white) or liver metastases (grey) was assessed by considering localization of immunopositive cells in an overview picture of each tumoral lesion (inside, margin or evenly distributed between inside and margin). Then, percentage of PD-L1 (B), CD68 (E), CD163 (H) and CD8 (K) immunopositive cells within tumoral lesions was determined. Finally, localization of the highest frequencies (peak category) for PD-L1 (C), CD68 (F), CD163 (I) and CD8 (L) immunopositive cells was determined. For percentage of immunopositive cells, ten fields of view (FoV) at the invasion front and center (at least one FoV away from invasion front) of the primary tumor or metastasis were evaluated according to the following classification: PD-L1 and CD8: 0%, <1% and >1% immunopositive cells or CD68 and CD163: 0%, <10%, 11-50% or >50% immunopositive cells. Two-way ANOVA with Tukey’s multiple comparison test and One-way ANOVA with Tukey’s multiple comparison test. n=4. * = p<0.05; ** = p<0.01; *** = p<0.001.