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. Author manuscript; available in PMC: 2023 Jul 12.
Published in final edited form as: Cell Rep. 2023 Mar 13;42(3):112245. doi: 10.1016/j.celrep.2023.112245

KEY RESOURCES TABLE.

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rabbit anti-PCDH15-CD2 Webb et al.30 N/A
Rabbit anti-LHFPL5/TMHS Xiong et al.23 N/A
Rabbit anti-PMCA2 Thermo Fisher Scientific Cat# PA1-915, RRID:AB_2243199
Rabbit anti-HA Cell Signaling Cat#: 3724; RRID:AB_1549585
Rabbit anti-MYC Cell Signaling Cat#: 2278; RRID:AB_490778
Mouse anti-HA Cell Signaling Cat#: 2367S; RRID: AB_10691311
Mouse anti-MYC Cell Signaling Cat#: 2276S; RRID: AB_331783
Mouse anti-FLAG Cell Singaling Cat#: 8146S; RRID: AB_10950495
Rabbit anti-FLAG Cell Signaling Cat#: 14793S; RRID: AB_2572291
Goat anti-Rabbit IgG F(ab’)2, Alexa Fluor 488 Invitrogen Cat#: A-11070; RRID:AB_2534114
Goat anti-Rabbit IgG F(ab’)2, Alexa Fluor 555 Invitrogen Cat#: A-21430; RRID:AB_2535851
Goat anti-Mouse IgG F(ab’)2, Alexa Fluor 647 Invitrogen Cat#: A-21237; RRID:AB_2535806
EZ-View Red HA Affinity Gel Sigma Cat#: E6779-1ML; RRID:AB_10109562
EZ View Red anti-c-MYC Affinity Gel Sigma Cat#: E6654-1ML; RRID:AB_10093201
Veriblot Abcam Cat#: ab131366
Veriblot anti-Mouse Abcam Cat#: ab131368
Chemicals, peptides, and recombinant proteins
Cas9 Protein PNA Bio Cat#: CP01-20
Tris-HCl Sigma Cat#: T5941
EDTA Sigma Cat#: E5134
DMEM+Glutamax medium Gibco Cat#: 10,569–010
Antibiotic-Antimycotic supplement Gibco Cat#: 15,240–062
DMEM/F12 medium Gibco Cat#: 11,330–032
Fetal Bovine Serum, Heat Inactivated Sigma Cat#: F4135
Ampicillin Sigma Cat#: A9518
Sodium Chloride VWR Cat#: 0241
Sodium phosphate monobasic Sigma Cat#: S8282
Potassium Chloride Fisher Scientific Cat#: P217
Calcium Chloride Fisher Scientific Cat#: M13841
Magnesium Chloride Sigma Cat#: M8266
Glucose Sigma Cat#: G7021
HEPES Sigma Cat#: H4034
BAPTA Sigma Cat#: A4926
EGTA VWR Cat#: 0732
Magnesium-ATP Sigma Cat#: A9187
Sodium-GTP Sigma Cat#: G8877
Cesium Chloride Sigma Cat#: 203025
DMSO Sigma Cat#: D2650
Paraformaldehyde Electron Microscopy Sciences Cat#: 15714
1X HBSS medium Gibco Cat#: 14175095
Normal Goat Serum, Heat Inactivated Gemini Biosciences Cat#: 100–109
Triton X-100 Sigma Cat#: T9284
Alexa Fluor 555 Phalloidin Life Technologies Cat#: A34055
Alexa Fluor 488 Phalloidin Life Technologies Cat#: A12379
Prolong Gold ThermoFisher Cat#: P10144
Lipofectamine 3000 ThermoFisher Cat#: L3000015
Tris/Tham Fisher Cat#: T370-3
NP-40 Sigma Cat#: I3021
Sodium Deoxycholate Sigma Cat#: 6750
Sodium Dodecyl Sulfate Sigma Cat#: 75746
Complete Mini Protease Inhibitor Tablet, EDTA-free Roche Cat#: 11836170001
4× Laemmli Sample Buffer Biorad Cat#: 1610747
2-Mercaptoethanol Biorad Cat#: 1610710
Novex™ WedgeWell™ 4 to 20%, Tris-Glycine, 1.0 mm, Mini Protein Gel Life Technologies Cat#: XP04202BOX
10× Tris/Glycine/SDS Biorad Cat#: 1610732
10× Tris/Glycine Buffer for Western Blots and Native Gels Biorad Cat#: 1610734
Methanol VWR Cat#: BDH1135-4LP
ECL Prime Blocking Reagent GE Cat#: RPN418
Tween 20 Sigma Cat#: P7949
Clarity Western ECL Substrate Bio-Rad Cat#: 1705060
BSA, fatty acid free Sigma Cat#: A8806
Experimental models: Cell lines
Human: HEK-293 ATCC Cat#: CRL-1573
Experimental models: Organisms/strains
Lhfpl5 −/− (Tmhs tm1kin) Longo-Guess et al.25 MGI:3718680
Lhfpi3−/− mice Tang et al.27 RRID:MMRRC_032433-UCD
Lhfpl5-Ndel3-I13L mice This paper N/A
C57BL/6J mice Jackson Laboratories Cat#: 000664; RRID:IMSR JAX:000,664
ICR mice Envigo Cat#: Hsd:ICR (CD-1)
Oligonucleotides
Target-specific crRNAs This paper/IDT N/A
tracrRNA Dharmacon Cat#: U-002005-05
Target-specific ssDNA This paper/IDT Ultramer DNA Oligos N/A
Recombinant DNA
pN3-TMIE-MYC Zhao et al.14 N/A
pN3-HA-CIB2 Liang et al.34 N/A
pCAGEN-TMC1-MYC Cunningham et al.15 N/A
pcDNA-PCDH15-CD2 Xiong et al.23 N/A
pCMV-LHFP-MYC Origene MR202027
pCMV-LHFPL1-MYC Origene MR202519
pCMV-LHFPL2-MYC Origene MR202553
pcDNA-LHFPL2 This paper N/A
pcDNA-LHFPL3 This paper N/A
pcDNA-LHFPL3-HA This paper N/A
pcDNA-LHFPL3-MYC This paper N/A
pcDNA-LHFPL3-FLAG This paper N/A
pcDNA-LHFPL4 This paper N/A
pcDNA-LHFPL4-MYC This paper N/A
pcDNA-LHFPL5 Xiong et al.23 N/A
pcDNA-LHFPL5-HA This paper N/A
REAGENT or RESOURCE SOURCE IDENTIFIER
pcDNA-LHFPL5-MYC This paper N/A
pcDNA-LHFPL5-FLAG This paper N/A
pcDNA-L5-L3 This paper N/A
pcDNA-L5-L3-HA This paper N/A
pcDNA-L5-L3-FLAG This paper N/A
pcDNA-L3-L5 This paper N/A
pcDNA-L3-L5-HA This paper N/A
pcDNA-L3-L5-FLAG This paper N/A
pcDNA-NTL5-L3 This paper N/A
pcDNA-NTL5-L3-HA This paper N/A
pcDNA-NTL5-L3-FLAG This paper N/A
pcDNA-NTL3-L5 This paper N/A
pcDNA-NTL3-L5-HA This paper N/A
pcDNA-NTL3-L5-FLAG This paper N/A
pcDNA-L5-Ndel3 This paper N/A
pcDNA-L5-Ndel3-HA This paper N/A
pcDNA-L5-I13L This paper N/A
pcDNA-L5-I13L-HA This paper N/A
pcNDA-L5NcytoTM1 This paper N/A
pcNDA-L5NcytoTM1-HA This paper N/A
pcNDA-L5NcytoTM1-FLAG This paper N/A
pcNDA-L5NcytoTM1Lp1 This paper N/A
pcDNA-L5NcytoTM1Lp1-HA This paper N/A
pcDNA-L5NcytoTM1Lp1-FLAG This paper N/A
pCAGEN-IRES-GFP Addgene Cat#: Addgene #11159; RRID:Addgene_11159
pCAGEN-MYC-TMC1-IRES-GFP This paper N/A
pCAGEN-MYC-TMC2-IRES-GFP This paper N/A
pCAGEN-MYC-TMC3-IRES-GFP This paper N/A
pCAGEN-MYC-TMC1-H1-TMC3-IRES-GFP This paper N/A
pCAGEN-MYC-TMC1-H2-TMC3-IRES-GFP This paper N/A
pCAGEN-MYC-TMC1-H3-TMC3-IRES-GFP This paper N/A
pCAGEN-MYC-TMC1-H1/2-TMC3-IRES-GFP This paper N/A
pN3-PAC-HA This paper N/A
pN3-PAC-TMC1-HA This paper N/A
pN3-PAC-TMC1-H1 -TMC3-HA This paper N/A
pN3-PAC-TMC1-H2-TMC3-HA This paper N/A
pN3-PAC-TMC1-H1/2-TMC3-HA This paper N/A
pN3-PAC-TMC1-H3-TMC3-HA This paper N/A
Software and algorithms
Deltavision Elite Software (SoftWoRx Resolve 3D) GE https://www.gelifesciences.com/en/ee/shop/deltavision-elite-high-resolution-microscope-p-04420
Imaris 9.7 Oxford Instruments https://imaris.oxinst.com/packages
Igor pro 7 WaveMetrics https://www.wavemetrics.com/
TDT System 3 Tucker-Davis Technology https://www.tdt.com/products/
Patchmaster 2.35 HEKA http://www.heka.com/downloads/downloads_main.html
Adobe Illustrator 2020 Adobe https://www.adobe.com/
Adobe Photoshop 2020 Adobe https://www.adobe.com/