TABLE 1.

Various Pullulanases their substrate and products.

Sl. No.	Accepted name	EC number	Other names	Systematic name	Bond hydrolyzed	Substrates	Products	References
1	P-I	3.2.1.41	α-dextrin 6 glucanohydrolase, pullulan α-1,6-glucanohydrolase, pullulanase, α-dextrin endo-1,6-α-glucosidase	Pullulan 6-α-glucanohydrolase	α-(1,6)- D-glucosidic linkages	pullulan, amylopectin, glycogen, α-and β-limit dextrinsother branched oligosaccharide	α-and β-limit dextrins, Maltose, maltotriose, and linear oligosaccharides	[Lee and Whelan, 1966; Bender and Wallenfels, 1966; Man
			R-enzyme; pullulan 6-glucanohydrolase					Bertoldo and Antranikian, 2002; Guo et al., 2018
2	P- II	3.2.1.41	Amylopullulanase		α-(1,6) and α-(1,4) D-glucosidic linkages	Pullulan, Poly and oligo saccharides, starch, amylopectin	Maltotriose, glucose, maltose	Duffner et al., 2000; Roy and Gupta, 2004; Lévêque et al., 2000
3	PH-1	3.2.1.135	Neopullulanase	pullulan 4-D-glucanohydrolase	α-(1,4) D-glucosidic linkages	Pullulan	Panose	Ara et al., 1995; Imanaka and Kuriki, 1989; Sunna et al., 1997
4	PH-II	3.2.1.57	Isopullulanase	pullulan 4-glucanohydrolase (isopanose-forming)	α-(1,4) D-glucosidic linkages	Pullulan, Panose	Isopanose, isomaltose and glucose
5	PH-III		-		α-(1,6) and α-(1,4) D-glucosidic linkages	Pullulan, Starch, amylose and amylopectin	Panose, maltose and maltotriose	Niehaus et al. (2000)

Symbols used: P-I, Pullulanase type I, P-II, Pullulanase type II, PH-I, Pullulan hydrolase type I, PH-II, Pullulan hydrolase type II, PH-II, Pullulan hydrolase type III.