TABLE 2.
Source microorganisms and properties of pullulanase.
| Types of pullulanase | Organisms | Enzyme properties | References | ||
|---|---|---|---|---|---|
| Optimum T (˚C) | OptimumpH | Molecular weight (kDa) | |||
| Bacillus stearothermophilus | 60–65 | 6.0 | 62 | Kuriki et al. (1988) | |
| Pullulanase Type I | FervidobacteriumpennavoransVen5 | 65–85 | 6.0 | 83, 93 | Bertoldo et al. (1999) |
| Paenibacilluspolymyxa | 35 | 6.0 | 96 | Wei et al. (2015) | |
| Thermusculdophilus GK-24 b | 75 | 5.5 | 65 | Kim et al. (1999) | |
| Pullulanase type II | Thermoanaerobacterethanolicus | 90 | 5.5 | - | Mathupala et al. (1993) |
| Pyrococcusfuriosus | 50 | 6.0 | 90 | Zona et al. (2004) | |
| Bacillus cereus | 55 | 6.0 | NA | Ling et al. (2009) | |
| Pyrococcuswoesei | 100 | 6.0 | 90 | Rüdige et al. (1995) | |
| Thermotoga maritima | 90 | 7.5 | 58 | Domań-Pytka and Bardowski (2004) | |
| Streptococcus infantarius | 30–45 | 6.8–8.0 | 246.3 | Rodrigues et al. (2013) | |
| Lactobacillus cripatus | 37 | 3.5–4.0 | - | Woolston et al. (2021) | |
| Pullulan hydrolase type I | Alicyclobacillus acidocaldarius | 55 | 5.5 | 66 | Domań-Pytka and Bardowski (2004) |
| Bacillus stearothermophilus | 60–70 | - | 69 | Domań-Pytka and Bardowski (2004) | |
| Thermoactinomyces vulgaris | 40 | 5–6 | 65 | Domań-Pytka and Bardowski (2004) | |
| Pullulan hydrolase type II | Aspergillus niger | 30–40 | 3.4–4.0 | - | Sakano et al. (1971) |
| Pullulan Hydrolase type III | Thermococcus aggregans | 100 | 6.5 | 83 | Niehaus et al. (2000) |
| Thermococcus kodakarensis | 95–100 | 3.5–4.2 | 84 | Ahamad et al., 2013; Toor et al., 2020 | |
Symbols used: NA, data not available; T-temperature.