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. 2023 Jul 10;33(13):2646–2656.e4. doi: 10.1016/j.cub.2023.05.028

Figure 3.

Figure 3

Quantification of MTAL1-deficient M. rhizoxinica inside R. microsporus

(A) Apo-symbiotic R. microsporus was individually co-incubated with SYTO9-stained M. rhizoxinica wild type (M1WT), M. rhizoxinica Δmtal1, M. rhizoxinica Δmtal1 pBBR∅, or M. rhizoxinica Δmtal1 pBBR-mtal1 for 48 h. After fluorescence microscopy at 485/498 nm, the integrated density per bacterial cell number was calculated for both time points (24 and 48 hpi). Dots represent three independent replicates (n = 3 biological replicates) ± SEM (gray bars). One-way ANOVA with Tukey’s multiple comparison test (∗p < 0.05, Data S2A–S2D).

(B) The integrated density per cell number was measured for each individual type of hyphae. n = 3 biological replicates ± SEM. One-way ANOVA with Tukey’s multiple comparison test (∗∗∗p < 0.0002, Data S3A–S3D).

(C) Fluorescence microscopy images showing the accumulation of M. rhizoxinica Δmtal1 (green) in populated hyphae of R. microsporus (blue). Septa are indicated by an arrow head. Scale bars: 10 μm. See also Figures S3C–S3E and S4.