Bacterial and virus strains |
|
Mycetohabitans rhizoxinica |
This study |
HKI-454 |
Electrocompetent Escherichia coli TOP10 |
Invitrogen |
CAT#C404052 |
|
Chemicals, peptides, and recombinant proteins |
|
Potato Dextrose Agar |
Becton, Dickinson & Co. |
CAT#213400 |
Glycerol |
Roth |
CAT#6967.1 |
Yeast Extract |
Becton, Dickinson & Co. |
CAT#288620 |
K2HPO4
|
Roth |
CAT#P749.2 |
KH2PO4
|
Roth |
CAT#3904.1 |
C6H7NaO |
Roth |
CAT#HN13.3 |
(NH4)2SO4
|
Roth |
CAT#9212.1 |
Mg2SO4
|
Roth |
CAT#P027.1 |
Standard Nutrient Agar I |
Merck |
CAT#1.03864.0500 |
Phusion® High-Fidelity PCR Master Mx |
New England Biolabs |
CAT#M0530L |
Apramycin |
Sigma |
CAT#A2024 |
SpeI |
New England Biolabs |
CAT#R3133L |
KpnI |
New England Biolabs |
CAT#R3142L |
OneTAQ® Quick-Load® 2x Master Mix |
New England Biolabs |
CAT#M0486L |
Ethidium bromide |
New England Biolabs |
CAT#E1510 |
NdeI |
New England Biolabs |
CAT#R0111L |
AflII |
New England Biolabs |
CAT#R0520L |
BstBI |
New England Biolabs |
CAT#R0519L |
Chloramphenicol |
Roth |
CAT#3886.1 |
Potato Dextrose Broth |
Becton, Dickinson & Co. |
CAT#254920 |
NaCl |
Roth |
CAT#3957.1 |
SYTO™ 9 |
Invitrogen |
CAT#S34854
|
LIVE/DEAD™ BacLight™ Bacterial Viability Kit |
Invitrogen |
CAT#L7012 |
Propidium iodide |
Invitrogen |
CAT#P21493 |
Calcofluor white |
Sigma |
CAT#18909 |
GeneRuler™ DNA Ladder Mix |
Thermo Scientific |
CAT#SM0333 |
T4 DNA Ligase |
New England Biolabs |
CAT#M0202L |
Ciprofloxacin |
Sigma |
CAT#17850 |
|
Critical commercial assays |
|
Monarch® DNA Gel Extraction Kit |
New England Biolabs |
CAT#T1020L |
NEBuilder® HiFi DNA Assembly Cloning Kit |
New England Biolabs |
CAT#E2621L |
MasterPure™ DNA Purification Kit |
Biozym |
CAT#160502 |
Monarch® Plasmid Miniprep Kit |
New England Biolabs |
CAT#T1010L |
|
Experimental models: Organisms/strains |
|
Rhizopus microsporus |
Jena Microbial Resource Collection |
ATCC 62417 |
|
Oligonucleotides |
|
See Data S6 for a list of oligonucleotides |
|
N/A |
|
Recombinant DNA |
|
Plasmid pZU17 |
This paper |
N/A |
Plasmid pBBR-mtal1
|
This paper |
N/A |
Plasmid pBBR∅ |
This paper |
N/A |
Plasmid pGL42 |
Lackner et al.16,43
|
N/A |
|
Software and algorithms |
|
Zen Blue |
Zeiss |
N/A |
Zen Black |
Zeiss |
N/A |
Fiji |
Schindelin et al.51
|
N/A |
GraphPad Prism 9.0 |
GraphPad Software |
N/A |
|
Other |
|
M. rhizoxinica genome |
Lackner et al.16,43
|
Genbank Accession #GCA_000198775.1 |
NanoDrop™ One |
Thermo Scientific |
N/A |
Eporator Electroporator |
Eppendorf |
N/A |
Quantum ST5 Xpress Gel Imager |
Vilber Lourmat |
N/A |
Bacterial-Fungal-Interaction devicesDevices |
This paper |
N/A |
CASY Cell Counter |
OMNI Life Science |
N/A |
Fluorescence Spinning Disc Microscope |
Zeiss |
Axio Observer platform with Cell Observer SD |
Confocal Laser Scanning Microscope |
Zeiss |
LSM 710 |
Fluorescence Widefield Microscope |
Zeiss |
Elyra7 |