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. 2023 Jul 10;33(13):2646–2656.e4. doi: 10.1016/j.cub.2023.05.028
REAGENT or RESOURCE SOURCE IDENTIFIER
Bacterial and virus strains

Mycetohabitans rhizoxinica This study HKI-454
Electrocompetent Escherichia coli TOP10 Invitrogen CAT#C404052

Chemicals, peptides, and recombinant proteins

Potato Dextrose Agar Becton, Dickinson & Co. CAT#213400
Glycerol Roth CAT#6967.1
Yeast Extract Becton, Dickinson & Co. CAT#288620
K2HPO4 Roth CAT#P749.2
KH2PO4 Roth CAT#3904.1
C6H7NaO Roth CAT#HN13.3
(NH4)2SO4 Roth CAT#9212.1
Mg2SO4 Roth CAT#P027.1
Standard Nutrient Agar I Merck CAT#1.03864.0500
Phusion® High-Fidelity PCR Master Mx New England Biolabs CAT#M0530L
Apramycin Sigma CAT#A2024
SpeI New England Biolabs CAT#R3133L
KpnI New England Biolabs CAT#R3142L
OneTAQ® Quick-Load® 2x Master Mix New England Biolabs CAT#M0486L
Ethidium bromide New England Biolabs CAT#E1510
NdeI New England Biolabs CAT#R0111L
AflII New England Biolabs CAT#R0520L
BstBI New England Biolabs CAT#R0519L
Chloramphenicol Roth CAT#3886.1
Potato Dextrose Broth Becton, Dickinson & Co. CAT#254920
NaCl Roth CAT#3957.1
SYTO™ 9 Invitrogen CAT#S34854
LIVE/DEAD™ BacLight™ Bacterial Viability Kit Invitrogen CAT#L7012
Propidium iodide Invitrogen CAT#P21493
Calcofluor white Sigma CAT#18909
GeneRuler™ DNA Ladder Mix Thermo Scientific CAT#SM0333
T4 DNA Ligase New England Biolabs CAT#M0202L
Ciprofloxacin Sigma CAT#17850

Critical commercial assays

Monarch® DNA Gel Extraction Kit New England Biolabs CAT#T1020L
NEBuilder® HiFi DNA Assembly Cloning Kit New England Biolabs CAT#E2621L
MasterPure™ DNA Purification Kit Biozym CAT#160502
Monarch® Plasmid Miniprep Kit New England Biolabs CAT#T1010L

Experimental models: Organisms/strains

Rhizopus microsporus Jena Microbial Resource Collection ATCC 62417

Oligonucleotides

See Data S6 for a list of oligonucleotides N/A

Recombinant DNA

Plasmid pZU17 This paper N/A
Plasmid pBBR-mtal1 This paper N/A
Plasmid pBBR∅ This paper N/A
Plasmid pGL42 Lackner et al.16,43 N/A

Software and algorithms

Zen Blue Zeiss N/A
Zen Black Zeiss N/A
Fiji Schindelin et al.51 N/A
GraphPad Prism 9.0 GraphPad Software N/A

Other

M. rhizoxinica genome Lackner et al.16,43 Genbank Accession #GCA_000198775.1
NanoDrop™ One Thermo Scientific N/A
Eporator Electroporator Eppendorf N/A
Quantum ST5 Xpress Gel Imager Vilber Lourmat N/A
Bacterial-Fungal-Interaction devicesDevices This paper N/A
CASY Cell Counter OMNI Life Science N/A
Fluorescence Spinning Disc Microscope Zeiss Axio Observer platform with Cell Observer SD
Confocal Laser Scanning Microscope Zeiss LSM 710
Fluorescence Widefield Microscope Zeiss Elyra7