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. 2023 Jul 12;9(28):eadg5175. doi: 10.1126/sciadv.adg5175

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Fig. 4. — (A) Comparison of avian (H9N2-PB2-627E) and mammalian-signature (H9N2-PB2-627K) vRNP assembly in TKO cells reconstituted with chANP32A or huANP32A/B. TKO cells were transfected with the indicated plasmids for 24 hours; cell lysates were immunoprecipitated with anti-Flag M2 Magnetic Beads and then analyzed by Western blotting. (B) Comparison of the effect of H9N2-NS2 on the H9N2 vRNP assembly in TKO cells reconstituted with either huANP32A/B or chANP32A. TKO cells were transfected with the indicated plasmids. After anti-Flag precipitation at 24 hours after transfection, the indicated proteins were analyzed by Western blotting. (C) Comparison of the effect of H9N2 NS2 on the H9N2 vRNP assembly in TKO cells without reconstitution of ANP32 proteins. Experiments were performed as in (B), except that the ANP32 constructs were not transfected. Following anti-Flag precipitation at 24 hours after transfection, the indicated proteins were analyzed by Western blotting. (D to G) GST affinity isolation analysis of the complex formation of NS2 with PA (D), PB1 (E), PB2 (F), or NP (G). In (A) to (G), three independent experiments were performed, with consistent results in each experiment.