Fig 2. Growth characteristics under ideal conditions.

Shown is the ability of the various mutants listed at the top of the Figure to grow in a standard nutrient rich medium (THY medium) cultured under oxygenated (Shaken, aerobic) or oxygen-limited (Static, anaerobic) conditions, as listed. The top panel (Broth Grown) presents images of culture tubes follow overnight culture in liquid THY medium. The degree of turbidity corresponds to the amount of growth, which was quantitated by spot plating on solid media (THY plates) as shown in the bottom panel (Plated) under the atmospheric conditions indicated. The spot plate data presented are a montage assembled from individual images collected from the same experiment, that are delineated by the boxes numbered at the bottom of the Figure. Culture conditions are described in detail in the Materials and Methods. Strains are as described in Fig 1.