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Troubleshooting guide for profiling the specificity of 2-PCA
| Problem | Possible Causes | Solutions |
|---|---|---|
| Few or no peptides identified by LC-MS/MS | Inefficient de-salting Contamination with polymer or detergent |
Ensure the pH of the peptide sample is below 3 before apply to C18 and that the resin is equilibrated properly. Use only non-autoclaved plastics. Do not store buffers in glassware washed with detergents. |
