Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (C. elegans) | lgg-1 | Wormbase | WBGene00002980 | |
| Strain, strain background (C. elegans) | N2 | CGC | Wild-type strain | |
| Genetic reagent (C. elegans) | DA2123 | CGC | adIs2122[gfp::lgg‐1;rol‐6(su1006)] | |
| Genetic reagent (C. elegans) | GK1057 | Sato and Sato, 2011 | Pspe‐11‐hsp‐6::GFP | |
| Genetic reagent (C. elegans) | HZ455 | CGC | him‐5(e1490) V; bpIs131[sepa‐1::gfp] | |
| Genetic reagent (C. elegans) | HZ1685 | CGC | atg‐4.1(bp501) | |
| Genetic reagent (C. elegans) | MAH247 | CGC | sqls25[atg‐18 p::atg‐18::gfp +rol‐6(su1006) ] | |
| Genetic reagent (C. elegans) | RD202 | Legouis lab | Is202[unc‐119(ed3)III;plgg‐1::GFP::LGG‐1 G‐>A] | |
| Genetic reagent (C. elegans) | lgg-1(Δ) | Mitani lab | NBRP: tm3489 | lgg‐1(tm3489) |
| Genetic reagent (C. elegans) | lgg-2(tm5755) | Mitani lab | NBRP: tm5755 | lgg‐2(tm5755) |
| Genetic reagent (C. elegans) | RD363; lgg-1(Δ112–123) | This paper | lgg‐1(pp22)dpy‐10(pp157) Legouis lab | |
| Genetic reagent (C. elegans) | RD367; lgg-1(G116A) | This paper | lgg‐1(pp65[G116A]) Legouis lab | |
| Genetic reagent (C. elegans) | RD368; lgg-1(Δ100–123) | This paper | lgg‐1(pp66) Legouis lab | |
| Genetic reagent (C. elegans) | RD420; lgg-1(G116AG117*) | This paper | lgg‐1(pp141[G116AG117stop]) Legouis lab | |
| Genetic reagent (C. elegans) | RD421; lgg-1(G116AG117A) | This paper | dpy-10(pp163)lgg-1(pp142[G116AG117A]) Legouis lab | |
| Genetic reagent (C. elegans) | RD425 | This paper |
dpy-10(pp163)lgg1(pp142)/+; SEPA-1::gfp Legouis lab |
|
| Genetic reagent (C. elegans) | RD435 | This paper |
lgg‐1(pp141[G116AG117stop]); atg‐18 p::atg‐18::gfp +rol‐6(su1006) Legouis lab |
|
| Genetic reagent (C. elegans) | RD436 | This paper |
lgg‐1(pp65[G116A]); atg‐18 p:: atg‐18::gfp +rol‐6(su1006) Legouis lab |
|
| Genetic reagent (C. elegans) | RD440 | This paper |
lgg‐1(pp141[G116AG117stop]); lgg‐2(tm5755) Legouis lab |
|
| Genetic reagent (C. elegans) | RD446 | This paper |
lgg‐1(pp65[G116A]); lgg‐2(tm5755) Legouis lab |
|
| Genetic reagent (C. elegans) | RD447 | This paper |
lgg‐1(tm3489); atg‐18 p::atg‐ 18::gfp +rol‐6(su1006) Legouis lab |
|
| Genetic reagent (C. elegans) | RD448 | This paper |
lgg‐1(pp65[G116A]); SEPA‐1::gfp Legouis lab |
|
| Genetic reagent (C. elegans) | RD449 | This paper |
lgg‐1(pp141[G116AG117stop]); SEPA‐1::gfp Legouis lab |
|
| Genetic reagent (C. elegans) | RD450 | This paper | lgg‐1(tm3489)II; SEPA‐1::gfp Legouis lab | |
| Strain, strain background (S. cerevisiae) | BY4742 | Euroscarf | Mat alpha ura3Δ0, his3Δ1, leu2Δ0, lys2Δ0 | |
| Genetic reagent (S. cerevisiae) | OC513 | YKO collection | BY4742, atg1::KanMX4 | |
| Genetic reagent (S. cerevisiae) | OC612 | YKO collection | BY4742, atg8::KanMX4 | |
| Genetic reagent (S. cerevisiae) | OC608‐OC609 | This paper | BY4742, atg8G116A Legouis lab | |
| Genetic reagent (S. cerevisiae) | OC610‐OC611 | This paper | BY4742, atg8G116A‐R117* Legouis lab | |
| Genetic reagent (S. cerevisiae) | OC613 | This paper | BY4742, pho8::pho8Δ60‐URA3KL Legouis lab | |
| Genetic reagent (S. cerevisiae) | OC614 | This paper | BY4742, atg1::KanMX4, pho8:: pho8Δ60‐URA3KL Legouis lab |
|
| Genetic reagent (S. cerevisiae) | OC615 | This paper | BY4742, atg8::KanMX4, pho8:: pho8Δ60‐URA3KL Legouis lab |
|
| Genetic reagent (S. cerevisiae) | OC616‐OC617 | This paper | BY4742, atg8G116A, pho8:: pho8Δ60‐URA3KL Legouis lab |
|
| Genetic reagent (S. cerevisiae) | OC618‐OC619 | This paper | BY4742, atg8G116A‐R117*, pho8:: pho8Δ60‐URA3KL Legouis lab |
|
| Strain strain background (E. coli) | OP50 | CGC | see Material and Methods | |
| Genetic reagent (E. coli) | JA-C32D5.9 | Open Biosystem | lgg‐1 RNAi feeding bacterial clone | |
| Genetic reagent (E. coli) | JA-C56C10.12 | Open Biosystem | epg‐5 RNAi feeding bacterial clone | |
| Genetic reagent (E. coli) | JA-Y55F3AM.4 | Open Biosystem | atg-3 RNAi feeding bacterial clone; | |
| Genetic reagent (E. coli) | JA-M7.5 | Open Biosystem | atg-7 RNAi feeding bacterial clone | |
| Genetic reagent (E. coli) | JA-W03C9.3 | Open Biosystem | rab-7 RNAi feeding bacterial clone | |
| Genetic reagent (E. coli) | JA- Y39G10AR.10 | Open Biosystem | epg-2 RNAi feeding bacterial clone | |
| Sequence-based reagent | CrRNA(s) | Paix et al., 2015 |
dpy-10 : 5’GCUACCAUAGGCACCACGAGGU UUUAGAGCUAUGCUGUUUUG3’ |
|
| Sequence-based reagent | CrRNA(s) | This paper |
lgg-1 Legouis lab 5’UACAGUGACGAAAGUGUG UAGUUUUAGAGCUAUGCUGUUUUG3’ |
|
| Sequence-based reagent | Repair template | Paix et al., 2015; | dpy-10 : 5’CACTTGAACTTCAATACGGCAAGATGAGAATGACTGGAAACCGTACCGCATGCGGTGCCTATGGTAGCGGAGCTTCACATGGCTTCAGACCAACAGCCTAT3’ | |
| Sequence-based reagent | Repair template | This paper |
lgg-1 (G116A): Legouis lab 5’CTTTACATCGCGTACAGTGACGAAAGTGTCTACGCCGGAGAGGTCGAAAAGAAGGAATAAAGTGTCATGTAT3’ |
|
| Sequence-based reagent | Repair template | This paper |
lgg-1 (G116AG117 *): Legouis lab 5’TTCCTTTACATCGCCTACAGTGACGAAAGTGTGTACGCCTAAGAATTCGAAAAGAAGGAATAAAGTGTCATGTATTATCCG3’ |
|
| Sequence-based reagent | Repair template | This paper |
lgg-1 (G116AG117A): Legouis lab 5’TTCCTTTACATCGCCTACAGTGACGAAAGTGTGTACGCCGCAGAGGTCGAAAAGAAGGAATAAGAATTCAGTGTCATGTATTATCCGCCGACGAATGTGTATAC3’ |
|
| Sequence-based reagent | Universal tracrRNA | Dharmacon GE | U-002000–05 | 5’AACAGCAUAGCAAGUUAAAAUAAGGCU AGUCCGUUAUCAACUUGAAAAAGUGGC ACCGAGUCGGUGCUUUUUUU3’ |
| Peptide, recombinant protein | S. pyogenes Cas9 | Dharmacon | CAS11201 | Edit-R Cas9 Nuclease Protein, 1000 pmol |
| Antibody | anti‐LGG‐1 (rabbit polyclonal) | Springhorn and Hoppe, 2019 | Ab#3 WB (1:3000) | |
| Antibody | anti‐LGG‐1 (rabbit polyclonal) | Al Rawi et al., 2011 | Ab#1 WB (1:200) IF(1:100) | |
| Antibody | anti‐LGG‐2 (rabbit polyclonal) | Manil-Ségalen et al., 2014 | WB (1:200) IF (1:200) | |
| Antibody | anti‐Tubulin (mouse monoclonal) | Sigma | 078K4763 | WB (1:1000) |
| Antibody | anti-SEL-1 (rabbit polyclonal) | Hoppe’s lab | WB (1:8000) | |
| Antibody | anti-CDC-48.1 (rabbit polyclonal) | Hoppe’s lab | WB (1:5000) | |
| Antibody | Anti-Rabbit HRP (goat polyclonal) | Promega | W401B | WB (1:5000) |
| Antibody | Anti-mouse HRP (goat polyclonal) | Promega | W4021 | WB (1:10,000) |
| Antibody | anti-GABARAP (rabbit polyclonal) | Chemicon | AB15278 | IF (1:200) |
| Antibody | anti-GFP (mouse monoclonal) | Roche | 1814460 | IF (1:250) |
| Antibody | anti-mouse IgG Alexa Fluor488 (goat polyclonal) | Molecular Probes | A11029 | IF (1:500 to 1:1000) |
| Antibody | anti-rabbit IgG Alexa Fluor488 (goat polyclonal) | Molecular Probes | A110034 | IF (1:500 to 1:1000) |
| Antibody | anti-rabbit IgG Alexa Fluor568 (goat polyclonal) | Sigma-Aldrich | A11036 | IF (1:500 to 1:1000) |
| Antibody | anti-GFP (rabbit polyclonal) | Abcam | ab6556 | (Immunogold 1:10) |
| Antibody | anti-rabbit IgG (goat polyclonal) | Biovalley | 810.011 | Coupled to 10 nm colloidal gold particles (Immunogold 1:20) |
| Chemical compound, drug | EPON | Agar Scientific | R1165 | see Materials and methods |
| Chemical compound, drug | lead citrate | Sigma‐Aldrich | 15326 | see Materials and methods |
| Chemical compound, drug | LRWHITE | Electron Microscopy Sciences | 14381 | see Materials and methods |
| Peptide, recombinant protein | LC3 traps | Quinet et al., 2022 | Molecular traps for LGG-1 | |
| Commercial assay or kit | Super Signal Pico Chemiluminescent Substrate | Thermo Scientific | 34579 | see Materials and methods |
| Commercial assay or kit | NuPAGE 4%‐12% Bis‐ Tris gel | Life Technologies | NP0321BOX | see Materials and methods |
| Software, algorithm | ImageJ | http://imagej.nih.gov/ij | see Materials and methods | |
| Software, algorithm | Fidji | https://fiji.sc/ | see Materials and methods | |
| Software, algorithm | Prism | GraphPad | see Materials and methods | |
| Software, algorithm | R software | https://www.r-project.org/ | see Materials and methods | |
| Software, algorithm | Crispr | http://Crispr.mit.edu | see Materials and methods | |
| Software, algorithm | Crispor | http://crispor.org | see Materials and methods | |
| Other | MitoTracker Red CMXRos | Molecular Probes | M7512 | see Materials and methods |