Figure 5.

HCTZ caused hepatic macrophage polarization and inflammation through a TLR4-dependent mechanism

(A–E) Relative MyD88 (A), NF-κB (B), TNF-α (C), IL-1β (D), and IL-6 (E) expression in the livers of mice treated with HCTZ or vehicle control (n = 5–8 per group). ∗p < 0.05, ∗∗p < 0.01. Data are represented as mean ± SEM.

(F–K) Representative western blots (F) and quantification of MyD88 (G), NF-κB (H), TNF-α (I), IL-1β (J), and IL-6 (K) levels in the livers of mice treated with HCTZ or vehicle control (n = 3–4 per group). ∗p < 0.05. Data are represented as mean ± SEM.

(L) Representative H&E staining in the livers of mice treated with HCTZ or vehicle control.

(M and N) (M)Representative CD80 staining of hepatic F4/80⁺ macrophages in mice treated with HCTZ or vehicle control.

(N) Flow cytometry analysis of CD80⁺-F4/80⁺ macrophages (n = 3–4 per group). ∗p < 0.05. Data are represented as mean ± SEM.

(O and P) (O)Representative CD206 staining of hepatic F4/80⁺ macrophages in mice treated with HCTZ or vehicle control.

(P) Flow cytometry analysis of CD206⁺-F4/80⁺ macrophages (n = 3–4 per group). ∗p < 0.05. Data are represented as mean ± SEM.