Figure 4.

NMR characterization of the EvdS6 UDP-pentose products.A, ¹H NMR of the reaction mixture zoomed from the pentose anomeric protons to the xylose sugar protons (Full spectrum in Fig. S5). The ribose sugar protons of the two products, NAD⁺, and NADH are generally labeled. The xylose sugar protons are also labeled. B, ¹H NMR zoomed in on only the xylose sugar proton peaks from 4.0 ppm to 3.5 ppm. K refers to UDP-4”-keto-D-xylose and X refers to UDP-D-xylose. C, NMR correlations noted from 2D spectra including COSY, NOESY, and HMBC experiments are summarized. Numbering is associated with numbering in the spectra shown.