Figure 6.

Active site architecture of EvdS6 in the ligand-free and TDP-bound states.A, active site of EvdS6 in the ligand-free state. The active site residues of EvdS6 are represented as stick in cyan. In the ligand-free state, some residues of the unstructured active site loop become highly flexible as interpreted due to the lack of any traceable electron density for R273-G275. B, superimposed view of TDP-bound and ligand-free active sites of EvdS6. The residues of the TDP-bound active site are represented as a stick, cyan while residues of the ligand-free active site are represented as a semitransparent stick, cyan. NAD⁺ is shown in yellow and TDP is shown in magenta.