Extended Data Fig. 1. Characterization of COOLAIR promoter deletion lines and functional analysis of FRI-GFP.
a, Genomic sequences around the 322-bp promoter deletion in the ΔCOOLAIR-2 line. Notably, there is a 37-bp insertion at +5919. b, COOLAIR expression is eliminated in the COOLAIR promoter deletion lines. Shown is a biologically-independent repeat experiment of Fig. 1b. c–f, Relative FRI (c,e) and FLC (d,f) expression in the indicated seedlings. Transcript levels of FRI and FLC (spliced) were normalized to PP2A. Relative expression to FRI-Col is presented. Individual data points are shown. Error bars for s.d. of three biological replicates. g, Relative levels of spliced FLC transcripts upon 42-d cold exposure. 5-d post cold, return to warmth for 5 d after 42-d constant cold exposure. Error bars for s.d. of three biological replicates.