Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 May 24;619(7969):378–384. doi: 10.1038/s41586-023-06112-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 3 — a) Representative cryo-EM micrograph from a set of 6000 micrographs collected with Titan Krios electron microscope at 300 keV. Nucleosome particles in multiple orientations are visible. b) Representative 2D class averages showing nucleosomes. Many details in nucleosomes are visible in 2D class averages. c) Cryo-EM map of nucleosome from the entire dataset, refined to 3.1 Å. Angular distribution for nucleosome is shown on the right. d) Fourier shell correlation (FSC) curve showing the resolution of the map in c). e) Directional FSC plot showing uniform resolution in all directions. f) Classification of the LIN28B nucleosome dataset resulted in three classes of nucleosome like particles (nucleosome, unwrapped nucleosome and hexasome). Number of particles corresponding to each class is indicated. g) Classification of LIN28B nucleosome particles from f) showing that DNA protrudes on both sides of nucleosome in some classes.