Extended Data Fig. 9. Target insertions and deletions in the center of piRNA:target duplex are detrimental for PIWI slicing.

a,b, Change in pre-steady-state cleavage rate for mononucleotide target insertions (a) or target deletions (b). Target insertion data are for 16 or 40 targets: four insertion geometries for ten piRISCs (MILI and EfPiwi for pairing up to g26,or MIWI for pairing up to g30). Guide bulge data are for four or ten targets: one deletion geometry for ten piRISCs (MILI and EfPiwi for pairing up to g26 or MIWI for pairing up to g30). Box plots show IQR and median. c, Fraction of cleaved targets in FACS-purified mouse primary spermatocytes for pairing containing a single mononucleotide bulge in target or guide sequence. Box plots show IQR and median; 95% confidence interval was calculated with 10,000 bootstrapping iterations.