Figure 4.

TRPS1 knockdown in human adipogenic progenitors impairs differentiation of mature white adipocytes in vitro. (A) Relative mRNA expression of TRPS1 in white pre-adipocytes during days 0, 2, and 5 of differentiation in response to siRNA-mediated TRPS1 knockdown (KD) or non-targeting control (NT), as quantified by qPCR (B) Oil Red-O-stained images of mature white adipocytes at Day18 of differentiation in response to TRPS1 KD or NT. Scale bar is 200 ums. (C) Relative mRNA expression of Adiponectin (ADIPOQ), Leptin, and CIDEA in mature white adipocytes during Day 18 of differentiation in response to siRNA-mediated TRPS1 knockdown (KD) or non-targeting control (D) Relative mRNA expression of C/EBPα, PPARγ, ZFP423, and NR4A1 in white pre-adipocytes during days 0, 2, and 5 of differentiation in response to siRNA-mediated TRPS1 knockdown (KD) or non-targeting control. Results are expressed as relative mRNA expression (fold-change) normalized to the housekeeping gene (36B4). Gene expression analysis was performed using the ddCT method. Graphs are shown as Mean +/− S.E.M. One-Way ANOVA (4 A, 4D) or unpaired t-test (B, C) were applied. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)