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. 2023 Jul 12;13:11268. doi: 10.1038/s41598-023-38212-8

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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A flowchart describing the main steps of the OrganoidChip experiments (a), and various sources of image blurring and loss of data caused by lack of proper immobilization (b). (a) The organoids were cultured on multiwell plates and then exposed to DOX for 48 h. Subsequently, organoids were introduced to the OrganoidChip for immobilization followed by calcium transients (cardiac organoids) and live/dead imaging. (b) The figure has three rows, each one representing a mechanism that disrupts high-resolution imaging. The top row depicts drifting caused by natural convection within the well of a chamber slide over 5 s. The middle row illustrates how fast stage movements can cause blur when having short exposure times. Typically, larger momentums and shorter exposure times (shown as “ex” on the figure) result in greater blur. The bottom row shows how much a cardiac organoid can propel itself, or “swim”, in 15 s in a petri-dish during calcium transients imaging. Scale bars represent 200 µm.