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. 2023 Jul 12;14(7):417. doi: 10.1038/s41419-023-05933-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Hierarchical clustering of differentially “writers” expression in HNP1 and MES28 cells. B METTL3 expression in NSCs and GSCs was detected by western blot. C METTL3 expression in primary (P) and recurrent (R) GBM samples was detected by western blot. D METTL3 expression in primary (Pri.) and recurrent (Rec.) GBM samples was detected by qPCR. E GSCs were treated with control or METTL3 shRNA. LINC00839 expression was detected by qRT-PCR. **p < 0.001. F Total RNA m6A contents in LINC00839-knockdown GSCs was quantified by dot blot assays. G m6A enrichment in LINC00839 transcripts in control and METTL3-silencing cells using MeRIP-qPCR. H Primer-walking strategy. Five paired primers were designed according to the gene/exon–intron length. I MeRIP-qPCR assays using walking primers were performed to analyze the m6A-modification region. J MeRIP-qPCR assays to analyze the m6A-modification levels of LINC00839 in MES28 transfected with LINC00839 wildtype and its mutants expression. K MeRIP-qPCR assays to analyze the m6A-modification levels of LINC00839 in control or METTL3-depleted MES28 transfected with LINC00839 wildtype and its mutants expression.