Table 5.
Pathological features of alopecia areata and immunohistochemical expression of Decorin before and after treatment in comparison with healthy controls
| Group A n = 13 |
Group B n = 11 |
Group C n = 10 |
Group D n = 12 |
Control n = 13 |
P1b | P2b | P3b | P4b | P5b | P6b | P7b | P8 b | P9b | P10b | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Histopathological features: Follicles count | |||||||||||||||
| Total follicles 1 count | 13 ± 1.7 | 13.9 ± 1.64 | 14.6 ± 1.9 | 14.2 ± 1.8 | 22.7 ± 2.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.0001* | 0.0004* | 0.008* | 0.007* |
| Total follicles 2 count | 16.7 ± 3.9 | 19 ± 3 | 19.7 ± 2.6 | 17 ± 4.4 | 22.7 ± 2.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.002* | 0.3 | 0.9 | 0.01* |
| p value∞a | 0.07 | 0.01* | 0.04* | 0.2 | 0.9 | ||||||||||
| Anagen follicles 1 | 2.7 ± 2.1 | 2.5 ± 1.8 | 2.9 ± 2.2 | 2.9 ± 2.3 | 18.9 ± 2.3 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.0001* | 0.0002* | 0.0008* | 0.0004* |
| Anagen follicles 2 | 10.6 ± 6.1 | 14.6 ± 8 | 14.3 ± 7.4 | 12.6 ± 8.9 | 18.9 ± 2.3 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.03* | 0.07 | 0.9 | 0.5 |
| p value∞a | 0.05 | 0.01* | 0.01* | 0.01* | 0.9 | ||||||||||
| Telogen/catagen follicles 1 | 7.1 ± 2.4 | 6.6 ± 2.4 | 7.1 ± 2.6 | 6.9 ± 2.5 | 1.9 ± 1 | 0.9 | 0.6 | 0.9 | 0.9 | 0.9 | 0.9 | 0.0002* | 0.0001* | 0.0006* | 0.0002* |
| Telogen/Catagen follicles 2 | 2.4 ± 2.4 | 1.3 ± 1.8 | 2 ± 2.4 | 1.1 ± 2.3 | 1.9 ± 1 | 0.9 | 0.9 | 0.4 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.3 |
| p value∞a | 0.04* | 0.05 | 0.01* | 0.03* | 0.9 | ||||||||||
| Terminal hair 1 | 9.8 ± 4.3 | 9.1 ± 3.7 | 10 ± 4.6 | 9.8 ± 4.4 | 20.9 ± 2.1 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.0002* | 0.0002* | 0.0006* | 0.0003* |
| Terminal hair 2 | 13 ± 0.4.9 | 16 ± 6.8 | 16.3 ± 5.7 | 13.7 ± 7.6 | 20.9 ± 2.1 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.006* | 0.5 | 0.7 | 0.059 |
| p value∞a | 0.03* | 0.008* | 0.01* | 0.03* | 0.9 | ||||||||||
| Vellus hair1 | 4.6 ± 3.9 | 4.7 ± 3.2 | 4.6 ± 4.1 | 4.4 ± 3.3 | 2.9 ± 1.5 | 0.9 | 0.6 | 0.9 | 0.9 | 0.9 | 0.9 | 0.0002* | 0.0002* | 0.0007* | 0.0001* |
| Vellus hair2 | 3.8 ± 5.1 | 3.1 ± 1.6 | 3.8 ± 2.9 | 3.8 ± 3.4 | 2.9 ± 1.5 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 | 0.9 |
| p value∞a | 0.9 | 0.6 | 0.9 | 0.7 | 0.3 | ||||||||||
| Intensity of immunohistochemical expression of decorin before treatment N (%) | |||||||||||||||
| Negative = 0 | 2(15.4%) | 1(9%) | 0(0%) | 1(8.3%) | 0(0%) | ||||||||||
| Weak = 1 | 11(84.6%) | 10(91%) | 10(100%) | 11(91.6%) | 1(7.7%) | ||||||||||
| Moderate = 2 | 0(0%) | 0(0%) | 0(0%) | 0(0%) | 2(15.4%) | ||||||||||
| Strong = 3 | 0(0%) | 0(0%) | 0(0%) | 0(0%) | 10(76.9%) | ||||||||||
| Mean intensity ± SD | 0.84 ± 0.37 | 0.8 ± 0.4 | 1 ± 0 | 0.81 ± 0.38 | 2.7 ± 0.63 | 0.9 | 0.8 | 0.9 | 0.7 | 0.9 | 0.8 | 0.0001* | 0.0001* | 0.0001* | 0.0001* |
| Intensity of immunohistochemical expression of decorin after treatment N (%) | |||||||||||||||
| Negative = 0 | 0 | 0 | 0 | 0 | 0 | ||||||||||
| Weak = 1 | 3(23%) | 3(27%) | 3(30%) | 4(33%) | 1(7.7%) | ||||||||||
| Moderate = 2 | 6(46%) | 2 (18%) | 2 (20%) | 3(25%) | 2(15.4%) | ||||||||||
| Strong = 3 | 4 (31%) | 6 (55%) | 5 (50%) | 5(42%) | 10(76.9%) | ||||||||||
| Mean intensity ± SD | 2.1 ± 0.75 | 2.3 ± 0.9 | 2.2 ± 0.9 | 2.1 ± 0.9 | 2.7 ± 0.63 | 0.9 | 0.9 | 0.9 | 0.3 | 0.9 | 0.9 | 0.7 | 0.9 | 0.6 | 0.3 |
| p value∞a | 0.01* | 0.01* | 0.01* | 0.01* | 0.9 | ||||||||||
1 = before treatment, 2 = after treatment. N = number of cases with specific intensity in each group, p value ∞ (before &after treatment).P1(A&B), P2(A&C), P3(A&D), P4(B&C), P5(B&D), P6(C&D), P7, P8, P9, P10 (healthy controls &groups A,B,C&D respectively).Wilcoxon test a, Mann–Whitney test b, * = Statistical significance.