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. 2023 May 10;129(2):237–248. doi: 10.1038/s41416-023-02278-y

Fig. 3. NDRG1 promotes tumour proliferation and carcinogenesis in HCC.

Fig. 3

a Expression plasmids for NDRG1 gene were made in a PCDH vector. Data from western blotting showed the successful overexpression of NDRG1 (NDRG1 OE) in PLC/PRF5 cells. b Growth curves of PLC/PRF/5 cells stably expressing PCDH-NDRG1 or PCDH vector. c The clony formation assay were performed to determine the proliferation of PLC-NDRG1 OE and PLC-PCDH cells. d Expression plasmids for NDRG1 shRNAs were made in a PLKO.1 lentiviral vector. Successful knockdown of NDRG1 (shNDRG1#1, shNDRG1#2) in PVTT-1 cells was also confirmed. e Growth curves of PVTT-1 cells stably expressing PLKO.1-shNDRG1 or PLKO.1-mock. f The colony formation of PVTT-1 cells stably expressing PLKO.1-shNDRG1 or PLKO.1-mock. g Suppression of NDRG1 protein reduced tumour initiation induced by PVTT-1 cells in nude mice. 2.5 million PLKO.1-mock or PLKO.1-NDRG1 shRNA stably transfected PVTT-1 cells were injected into the dorsal flank of 6-week-old nude mice subcutaneously and tumour incidence was checked 5 days after injection. Black arrows showed the tumour masses.