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. 2023 May 10;129(2):237–248. doi: 10.1038/s41416-023-02278-y

Fig. 5. EpCAM is a functional target of NDRG1 in HCC.

Fig. 5

a PLKO.1-mock or PLKO.1-NDRG1 shRNA stably transfected PVTT-1 cells were subjected to flow cytometry analysis to quantify EpCAM, CD44 or CD133 positive cells. b–d EpCAM protein were increased in PVTT-1 cells transfected with NDRG siRNAs or shRNAs, while opposite effects were observed in NDRG1 overexpressed cells. e NDRG1 protein level was determined by western blot in EpCAM-positive and negative cells isolated from Huh7. γ-tubulin was used as a loading control. f, g The mRNA and protein levels of NDRG1 in PVTT-1 cells transfected with NDRG1 siRNAs. h Correlation graphs between NDRG1 and EpCAM protein in 34 patients with HCC. Representative IHC images of low or high NDRG1/EpCAM expression in HCC tissues (left). Pearson correlation analysis of NDRG1 and EpCAM protein expressions in 34 HCC samples (r = 0.501, **P = 0.0025, n = 34) (right).