Table 5. PCR reaction master mix.

Reagent	Amount	Final Concentration
Q5 high-fidelity 2× master mix	25 μL	1×
10 μM forward primer	2.5 μL	0.5 μM
10 μM reverse primer	2.5 μL	0.5 μM
Plasmid (pST1374-EGFP-boxC/D)/plasmid (pST1374-sec-OVA-3HA-MITD-boxC/D)	variable	0.4 ng/μL
Nuclease-free water	to 50 μL	/