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| Reagent | Amount |
|---|---|
| IVT product from step D2c | 20 μL |
| 10× Poly(A) polymerase reaction buffer | 10 μL |
| Poly(A) polymerase | 5 μL |
| Nuclease-free water | to 100 μL |
Note: The unpurified IVT product contains enough ATP; no extra ATP is necessary for the poly(A) tailing reaction.
