Time (min)	Flow rate (mL/min)	Solvent A	Solvent B
0	0.7	94	6
6	0.7	94	6
10	0.7	75	25
11	0.7	2	98
13	0.7	0	100
13.4	0.7	0	100
13.5	1.5	0	100
18.6	1.5	0	100
18.7	1.5	99.9	0.1
23	1.5	99.9	0.1
23.5	0.7	99.9	0.1
24	Stop